Plot the computed slope and intercept of the linear regression as a straight line overlaying your scattergram. The equation for a straight line is y = mx b, where m is the slope and b the intercept. 5
Notes
Since tyrosinase catalyzes the conversion of L-DOPA to dopachrome, this exercise measures the conversion of colorless DOPA to the dark orange dopachrome. Substrate and product are in a 1:1 ratio for this reaction, thus the amount of product formed equals the amount of substrate used. The optical density of dopachrome @475 nm is directly proportional to the intensity of orange color formation in solution (Beer-Lambert Law).
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