Mass Spectometry Data Analysis in Proteomics (Methods in Molecular Biology, Vol

Mass Spectometry Data Analysis in Proteomics (Methods in Molecular Biology, Vol 367)

Publisher: Humana Press Inc.
Number Of Pages: 320
Publication Date: 2007
Sales Rank: 333939
ISBN / ASIN: 158829563X
EAN: 9781588295637
Binding: Hardcover
Manufacturer: Humana Press Inc.
Studio: Humana Press Inc.

Mass Spectrometry Data Analysis in Proteomics is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. As MS is a high throughput technique, proteomic researchers must attend carefully to the associated field of data analysis, and this volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters. Several different types of MS data are discussed, followed by a description of optimal methods for conversion of raw data into peak lists for input to search engines. Choosing the most accurate and complete databases is emphasized, and a report of available sequence databases is included. Methods for assembling expressed sequence tags (ESTs) into assembled nonredundant databases are provided, along with protocols for further processing the sequences into a format suitable for MS data. Mass Spectrometry Data Analysis in Proteomics describes publicly available applications whenever possible.

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