In Vitro Transcription and Translation Protocols 2nd ed (Methods in Molecular Bi

In Vitro Transcription and Translation Protocols 2nd ed (Methods in Molecular Biology, Vol 375)

Publisher: Humana Press
Number Of Pages: 276
Publication Date: 2007
Sales Rank: 962814
ISBN / ASIN: 1588295583
EAN: 9781588295583
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.

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