PRINS and In Situ PCR Protocols (Methods in Molecular Biology, Vol 334)

PRINS and In Situ PCR Protocols (Methods in Molecular Biology, Vol 334)

Publisher: Humana Press
Number Of Pages: 264
Publication Date: 2006-04-01
Sales Rank: 2072139
ISBN / ASIN: 1588295494
EAN: 9781588295491
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press

This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.

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