Transmembrane Signaling Protocols, 2nd Edition (Methods in Molecular Biology vol

Transmembrane Signaling Protocols, 2nd Edition (Methods in Molecular Biology volume 332)

Publisher: Humana Press
Number Of Pages: 376
Publication Date: 2006-02-21
Sales Rank: 3340928
ISBN / ASIN: 158829546X
EAN: 9781588295460
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press

This fully updated and expanded second edition of Transmembrane Signaling Protocols demonstrates the use of such techniques as single cell imaging, read.freeduan.com bioluminecence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. The protocols follow the successful Methods in Molecular Biology