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Oligonucleotide Synthesis: Methods and Applications (Methods in Molecular Biolog

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Oligonucleotide Synthesis: Methods and Applications (Methods in Molecular Biology)

Publisher: Humana Press
Number Of Pages: 456
Publication Date: 2004-09-01
Sales Rank: 215448
ISBN / ASIN: 1588292339
EAN: 9781588292339
Binding: Hardcover
Manufacturer: Humana Press
Studio: Humana Press

Book Description:

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The read.freeduan.com applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

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http://rapidshare.com/files/36190912/Oligonucleotide_Synthesis-Methods_and_Applications_1588292339.rar


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