Oligonucleotide Synthesis: Methods and Applications (Methods in Molecular Biology)

Publisher: Humana Press Number Of Pages: 456 Publication Date: 2004-09-01 Sales Rank: 215448 ISBN / ASIN: 1588292339 EAN: 9781588292339 Binding: Hardcover Manufacturer: Humana Press Studio: Humana Press
Book Description:
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The read.freeduan.com applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
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