如何分析DNA测序结果

如何分析DNA测序结果

This guide includes an example of high quality sequence as well as many different problems that
occur with DNA sequences attained from the 3700 DNA Analyzer in the Plant-Microbe Genomics
Facility. For those figures that demonstrate a problem there is a (1) description of the problem, (2)
the most likely cause(s), and (3) one or more solutions.

If you have additional questions or comments about the figures below, then please do not hesitate to
contact the facility.


Figure 1 Electropherogram with high quality sequence. _______________________________________ 2
Figure 2 Electropherogram that demonstrates the limit of the resolution of the 3700 DNA Analyzer _3
Figure 3 Electropherogram with unincorporated nucleotide peaks.______________________________ 4
Figure 4 Electropherogram with mobility errors.______________________________________________ 5
Figure 5 Electropherogram that demonstrates a lack of extension products, i. e. no bands. ________ 6
Figure 6 Electropherogram that has multiple sequences.______________________________________ 7
Figure 7 Electropherograms that have homoN slippage. ______________________________________ 8
Figure 8 Electropherogram that has a strong stop. ___________________________________________ 9
Figure 9 Electropherogram that has a micro-air bubble or debris.______________________________ 10
Figure 10 Electropherogram that has "the spread".__________________________________________ 11
Figure 11 Electropherogram that has Primer N-1. ___________________________________________ 12