Genetics and Properties of Cellulases
Cellulases are enzymes which degrade the insoluble, abundant polymer cellulose. In order to perform this task bacteria, fungi, plants and insects have developed a variety of different systems with multiple cellulases. In this review the similarities and differences of these enzymes are summarized based on the burgeoning information gained in recent years from amino acid sequences, three dimensional structures and biochemical experiments. The independent cellulases of aerobic organisms are contrasted with the cellulosomes of anaerobic organisms. The ability of different enzymes to synergize with each other is discussed along with the role of the different types of enzymes in cellulose degradation.
1 Introduction 1.1 Eukaryotic and Prokaryotic Cellulases 1.2 Anaerobic Versus Aerobic Cellulases 2 Cellulase Domains 2.1 Catalytic Domain Families 2.2 Cellulose-Binding Domains 2.3 Cellulosome Structure 2.4 Linkers 3 Conservation of Cellulase Genes . 4 Multiple Cellulases . 4.1 Cellulase Synergism 4.2 Fragmentation Activity 5 Mechanisms of Cellulase Activity 6 Cellulase Regulation 7 Application of Cellulases 7.1 Engineering Cellulases References
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