Directed Enzyme Evolution: Screening and Selection Methods
ISBN: 158829286X
Title: Directed Enzyme Evolution: Screening and Selection Methods (Methods in Molecular Biology (Clifton, N.J.), V. 230,)
Author: Frances Hamilton Arnold (Editor), George Georgiou (Editor)
Publisher: Humana Press
Publication Date: 2003-05-16
Number Of Pages: 370
Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens.
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