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DNA转染
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· (PDF) (LTI)
· Transfection of Mammalian Cell (Calcium Phophate Method) (Botwell Lab)
· Transfection of Mammalian Cell (Electroporation Method) (Botwell Lab)
- Transfection of Mammalian Cells by the Calcium-Phosphate Method (Tyner Lab)
For transient or stable transfection
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Procedure for Transient or Stable Transfection of HeLa Cells Procedure for Transient or Stable Transfection of HeLa Cells In a six-well or 35 mm tissue culture plate, seed ~4 x 10^5 cells per well...
· (Life Technologies)
Procedure for Transient or Stable Transfection
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o ß-Gal Staining of Eukaryotic Cells In Vitro
· Selection of Transfected Suspension Cells (Hannun/Obeid Lab)
· (LTI)
Detailed and general protocol
· (Woodgett Lab)
· (Promega)
Chloramphenicol acetyltransferase (CAT), encoded by a bacterial drug-resistance gene, inactivates chloramphenicol by acetylating the drug at one or both of its two hydroxyl groups (1). This gene is not found in eukaryotes, and therefore eukaryotic cells contain no background of CAT activity.
· (Crawford Lab)
· (Promega)
For assaying beta-galactosidase activity in extracts prepared from cells transfected with beta-galactosidase reporter vectors.
- ß-gal staining of eukaryotic cells in vitro (Botwell Lab)
Cells previously transfected with a lac Z construct can be stained for b-galactosidase activity.
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· Assay for ß-Galactosidase Expression in situ (Life Technologies)
Assay for ß-Galactosidase Expression in situ Assay for ß-Galactosidase Expression in situ Transfect cell cultures with expression vector for ß-galactosidase, allow expression for 1-2 days...
· FAQs for Antibiotics (LTI)
tips for does and preparation of Geneticin (G418)
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