· (Bowtell Lab)
- In vitro Mutagenesis with dut ung single stranded DNA (Hahn Lab)
· Site-directed Mutagenesis using PCR (Lazo Lab)
PCR site-directed methods allow site-specific mutations to be incorporated in virtually any double-stranded plasmid; eliminating the need for M13-based vectors or single-stranded rescue.
· Site-directed mutagenesis (Dr. Chastain)
· (Bowtell Lab)
· (Jeff NEwman)
· (Promega)
For generation and selection of oligonucleotide-directed mutants
· (PMCI Research)
· (Bowtell Lab)
Nested Deletions using exonuclease-III and mung bean nuclease
· (Promega)
For construction of plasmid or M13 subclones containing progressive unidirectional deletions of inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation, transformation...
· (Fermentas)
For construction of plasmid or M13 subclones with nested unidirectional deletions. The method is applicable for DNA sequencing, mapping of boundaries of regions involved in genetic control, DNA/protein interaction sites, or protein domains.
· (Vesicle Trafficking)
(PDF) (Templenton Lab)
A PCR-based mutagenesis, based on Sarkar and Sommer, but with no ligation required.
· (PMCI Research)
· In vitro Mutagenesis Using Altered Sites (PMCI Research)
· (Crawford Lab)
(Promega)
Methylation analysis
· Bisulfite Treatment of DNA (Issa Lab)
· Methylation-Sensitive PCR (MSP) (Issa Lab)
· Bisulfite-PCR (for restriction and/or sequencing) (Issa Lab)
· Southern Blot Analysis (Issa Lab)
· Methylated CpG Island Amplification (MCA) (Issa Lab)
· DNA-Methyltransferase Assay (Issa Lab)
· Bisulfite Modification (TTO)
Modify DNA with bisulfite for methylation analysis
