用DAPI进行细胞染色DAPI的配置Stock solution of 1mg/ml in ddH2O; working solution of 0.25mg/ml to 50mg/ml in ddH2O or PGM. 1.Place sample on slide. 2.Add a few drops working solution. 3.Squash and view. Staining – DAPI Staining for Cells:Separately DAPI staining:%26amp;#8226; Wash the cells twice with PBS%26amp;#8226; Incubate with 1: 750 of stock DAPI for 5 min in dark%26amp;#8226; Wash 2 x 5 min with PBS in dark%26amp;#8226; Rinse with water for a few seconds%26amp;#8226; Air dry at RT in dark%26amp;#8226; Mount with antifade solution (Vectashield, Co#: H-1000, Vector Lab)5 %26amp;micro;l for each d12 mm coverslip, 25 %26amp;micro;l for square coverslip and 50%26amp;micro;l for full size coverslip%26amp;#8226; Seal the coverslip with nail polish%26amp;#8226; Store in 4 C up to 2 weeksDAPI stock%26amp;#8226; 4’, 6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) from Sigma (Cat# D-9542), 1 mg1mg/0.5 ml in H2O and stocked at –20 in the darkStaining with antifade mounting solution:%26amp;#8226; Wash the cells twice with PBS%26amp;#8226; Mount with antifade solution with DAPI (Vectashield, Co#: H-1200，Vector Lab, 1.5 %26amp;micro;g/ml) at 1:3 dilution with Vectashield H-1000Volume used as above%26amp;#8226; Store in 4 C up to 2 weeks
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