Holtfreter's Solution 显微注射鱼受精卵的保存液

Holtfreter's Solution 显微注射鱼受精卵的保存液

Holtfreter's Solution




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Source: based on Armstrong, J.B., Duhon, S.T., and Malacinski, G.M. (1989) Raising the axolotl in captivity. In J.B. Armstrong and G.M. Malacinski (eds.) Developmental Biology of the Axolotl. New York: Oxford University Press, pp. 220-227.

Modified protocol (but not recipe) 2/26/98 when we realized that MgSO4 and CaCl2 easily combine and precipitate out.


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100% Holtfreter's solution for dejellied embryos, before stage 38

NaCl 3.5 g
NaHCO3 0.2 g
KCl 0.05 g
MgSO4 stock solution 333