Greenberg’s Lab Tips 实验生物学圣经
标 题: Greenberg’s Lab Tips 实验生物学圣经
发信站: BBS 未名空间站 (Sat Mar 17 15:55:23 2007)
每日必读，实验失败必读，实验成功必读，设计实验前必读，读
paper前必读，读paper后必
读，深感字字箴言。
Greenberg’s Lab Tips 实验生物学圣经
(1)If the protocol works, don't change it (If it ain’t broke, don’t fix it
).
(2)If the protocol doesn’t work, change it.
(3)If a protocol requires a laborious step, there is usually a reason for it
– don’t try to avoid it (You are
not as smart as you think).
(4)Two hours of work today will save you two days of work tomorrow.
(5)Every new experiment needs a positive control and a negative control (
Corollary of(4),above).
(6)There are three types of experiments: One which yields interesting
results, no matter the outcome
(Type I), one which yields an interesting result, but only if it turns out
one way (Type II), and one which
yields ambiguous, uninterpretable results regardless of the outcome (Type
III).Take your pick.
(7)Label every tube and record every experiment.
(8)Assume nothing. A corollary of this is that opinions don’t matter, only
data does.
(9)Experiments often don’t work. Consider all the possible reason why an
experiment fails and
concentrate on the likeliest causes.
(10)Complicated, multi-step experiments never succeed unless they are
simplified. Break up each
experiment into its components, work on one at a time, and verify that each
component works before
proceeding on to the next(Take one step at a time).
(11)Don’t try to recreate the wheel. If someone else has successfully
performed the same experiment as
you plan to do, talk to her.
(12)Plan every experiment as if it will become a figure in a manuscript.
(13)If someone asks for your help, be as helpful as you can(What comes
around, goes around).
(14)It is more important to be accurate than prolific. It’s also helpful to
be prolific.
(15)If an experiment is not hypothesis-driven, then it can prove nothing.
(16)What matters in science is not what can possibly occur, but what
actually does occur.
(17)Read widely. The most creative insights come from applying knowledge
from outside one’s field.
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