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How many samples can be run per chip on the Experion system?
The Experion Pro260 chips can accommodate 10 protein samples and the RNA StdSens and HighSens chips can accommodate 12 or 11 RNA samples, respectively.
Experion Automated Electrophoresis — Run Time
How long does it take to run a chip on the Experion system?
Chips that are completely loaded with sample generally require 30 min per run. Less time is required for fewer samples.
Experion Automated Electrophoresis — Automated Steps
What steps of the electrophoresis process does the Experion system automate?
The Experion system combines electrophoresis, staining, destaining, imaging, band detection, and basic data analysis into a single, automated, 30 min step.
Experion Automated Electrophoresis — Detection Method
How are samples labeled in the Experion system? How are they detected?
In the Experion system, protein and RNA samples are labeled with a dye and are detected by laser-induced fluorescence.
Experion Automated Electrophoresis — Sample Labeling Process and Molecular Weight Determination
In the Experion system, how are protein and RNA samples labeled with dye? Does this labeling process affect the apparent size of proteins and protein MW determinations?
The Experion dye intercalates into the protein or RNA molecules by a process that does not appear to influence the migration patterns or apparent MW of proteins. Proteins in the Experion Pro260 ladder also bind the dye, so any effect is taken into account through normalization to the protein ladder.
Experion Automated Electrophoresis — Additional Equipment Required
What additional equipment/supplies are required to run the Experion system, aside from the Experion instrument and analysis kit?
Additional equipment require and sold by Bio-Rad are: the priming station for introducing gel into the chip, and the vortexer, which is required for all RNA applications. A computer with a USB port and cable and a 10 µl pipet are also required and must be supplied by the customer.
Software
Experion Automated Electrophoresis — Software Calculations
What calculations are performed automatically by Experion software?
For protein samples, Experion software automatically calculates MW, sample concentration (µg/ml), % of total sample, standard deviation, mean value, and % CV. For RNA samples, Experion software automatically calculates the total RNA concentration (ng/ml), mRNA concentration (ng/ml), rRNA contamination, 28S/18S or prokaryotic (16S) ratio, standard deviation, mean value, and % CV.
Experion Automated Electrophoresis — Data Display, General
How does Experion software display separation data?
Experion software displays data in a conventional electropherogram, or peak view, and in a simulated gel view.
Experion Automated Electrophoresis — Data Display, Simulated Gel View
What is the simulated gel view that is displayed by Experion software?
Experion software detects each protein or RNA moiety as it passes through the detector and creates a virtual gel image based on the migration of the protein or RNA relative to the standard.
Experion Automated Electrophoresis — Data Display, Results of Calculations
How are the results of calculations displayed by Experion software?
Calculations are presented in two different formats: the result table organizes data to compare information well-to-well, and the protein/RNA table organizes data to compare information (concentration/statistics/% total sample) of a particular protein or RNA of interest from the different wells.
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