全自动电泳疑问解答FAQs

Analysis Kits (Chips and Reagents)

Experion Automated Electrophoresis — Sample Types

What types of samples can the Experion separate and analyze?
The Experion automated electrophoresis system separates and analyzes protein and RNA (either mRNA or total RNA).

Experion Automated Electrophoresis — Analysis Kit Ordering Options

What are the various analysis kits available for the Experion system? What ordering configurations are available for these analysis kits?
The Experion system contains three analysis kits: The Pro260 kit for protein separation and analysis, the RNA StdSens kit for standard-sensitivity RNA detection, and the RNA HighSens kit for high-sensitivity RNA detection. All three analysis kits are available as 10- or 25-chip kits, for a total of 6 ordering options.

Experion Automated Electrophoresis — Consumables

What consumables will be sold separately for the Experion system?
Experion Protein and RNA chips (10-count) will be sold separately, as will electrode cleaning chips and reagents, DEPC-treated water, and spin filters.

Experion Automated Electrophoresis — Dynamic Range of Detection

What is the dynamic range of detection for the Experion system? What concentrations of proteins can be separated and detected with the Experion Pro260 analysis kit? What levels of RNA can be detected with the RNA HighSens and StdSens analysis kits?
The Experion Pro260 analysis kit separates and detects 2.5–2,000 ng/µl protein. The RNA HighSens analysis kit is designed for high-sensitivity RNA separation and detection down to 200–5,000 pg/µl RNA, and the RNA StdSens analysis kit detects 25–500 ng/µl RNA.

Experion Automated Electrophoresis — Sensitivity of Detection

What are the limits of protein and RNA detection with the Experion system?
The Experion system can detect 10 ng carbonic anhydrase (S/N > 3) with the Pro260 analysis kit, 5 ng/µl 18S rRNA in a total RNA sample (S/N > 3) with the RNA StdSens analysis kit, and 200 pg/µl 18S rRNA in a total RNA sample (S/N > 3) with the RNA HighSens analysis kit.

Experion Automated Electrophoresis — Sample Volumes

How much protein or RNA is required to run the Experion system? What sample volumes are required for the Experion Pro260, RNA HighSens, and RNA StdSens microfluidic chips?
The Experion chips require 4 µl of sample per well for protein analysis and 1 µl of sample per well for RNA analysis.

Experion Automated Electrophoresis — Minimum and Maximum Volumes in Chip Wells

What are the minimum and maximum volumes that the sample and gel wells on Experion chips can hold?
Sample wells can hold 4–10 µl (sample, ladder, and destain), and the gel well holds 7–18 µl gel matrix.

Experion Automated Electrophoresis — Shelf Life of Chips and Reagents

What is the shelf life of the chips and reagents in the Experion Pro260, RNA HighSens, and RNA StdSens analysis kits?
Reagents and chips of the Experion Pro260 analysis kit have a shelf life of 12 months. RNA chips have a shelf life of 15 months and the RNA reagents, 12 months.

Experion Automated Electrophoresis — Storage Temperature for Chips and Reagents

At what temperatures should the chips and reagents in the Experion Pro260, RNA HighSens, and RNA StdSens analysis kits be stored?
Protein and RNA chips may be stored at ambient temperature. Reagents should be stored at 4°C.

Electrophoresis, Priming, and Vortex Stations

Experion Automated Electrophoresis — Time Required for Chip Priming

How long does it take to prime a chip for the Experion system?
After initial setup, priming of the gel matrix takes 1–1.5 min in the automated priming station.

Experion Automated Electrophoresis — Number of Samples per Chip