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SDS
5 ml
TrisDTTNaOAc (pH5.2)NaOAcNaOAc (pH5.2)Tris base, 0.2M glycine12.5ml Tris base 7.5g glycine(MW:75.05) 100ml methanolTris base, 80 g NaCl; adjust pH to 7.6 with HCl (use at 1X).
Blocking buffernonfat milk and mix well.While strirring,add 0.15ml Tween-20(100%)BSA and mix well. While stirring, add 20
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