Immunohistochemical Staining (IHC) (Arcturus) For optimal LCM from IHC samples, it is necessary to minimize the amount of time the samples are incubated in an aqueous environment. Fewer and shorter incubation steps are recommended. For best results, use charged or poly-L lysine coated slides for mounting tissue sections. Tissues may not adhere through the entire IHC process on a plain glass slide.
Iron Stain-Perl's Method Demonstrating iron pigments in tissue which is normally found in small amounts in the bone marrow and the spleen.
Basement Membrane Stain-Jones Method The purpose of this procedure is to delineate the glomerular basement membrane.Methenamine silver methods rely on oxidation of carbohydrates to aldehydes.Silver ions from methenamine silver bind to carbohydrate components of the basement membrane and are reduced to visible metallic silver by the aldehyde groups.Toning is with gold chloride and any unreduced silver is removed by sodium thiosulfate.
NADH For Dehydrogenase Frozen Muscle Biopsy NADH is a stain used to indicate changes in the muscle resulting from rearrangement of the intermyofibrillar network, examples include: whorls, target fibers, central cores and moth-eaten fibers.
Fat Stain-Oil Red O on Frozen Sections the Oil Red O stain will demonstrate neutral lipids which are present in normal tissue and takes the form of small droplets within myofibers.
