| 利用PHOTOSHOP将两种荧光图象重叠，从而达到激光共聚焦效果，可以节省不少
操作步骤如下：
Photoshop Procedure to Colorize Immuno Images
Open Photoshop
Open both images (Cy3-red and Hoechest33258-blue)
1. Choose one image
Image—Mode—RGB Color
Image—Adjust—Brightness/Contrast.
Then Save As:
2. Choose other image
Image—Mode—RGB Color
Image—Adjust—Brightness/Contrast.
Then Save As:
3. Select one of the images—(say the Hoechest33258-blue)
Go to Channels
Click on Red—Go to Image—Adjust—Brightness/Contrast
Move both sliders to zero (or -100)
Click on Green—Go to Image—Adjust—Brightness/Contrast
Move both sliders to zero (or -100)
Save
4. Select the other image (Cy3-red)
Go to Channels
Click on Green—Go to Image—Adjust—Brightness/Contrast
Move both sliders to zero (or -100)
Click on Blue—Go to Image—Adjust—Brightness/Contrast
Move both sliders to zero (or -100)
Save
5. Select one of the images
Go to Layers
Double click on Background—Rename: Cy3-red
Save
6. Select the other image
Go to Layers
Double click on Background—Rename: Hoechest33258-blue
Save
7. Select All
Copy
8. Go to the other image
Paste
Change from “Normal” to “Screen” on Layers pallette.
Save As:
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