Immunization Protocol for Monoclonal Antibody Production
Antigens:
Adjuvant:
Schedule:
Day
Manipulation
Adjuvant
Site
0
Primary Immunization
CFA
s.c.
14
Boost #1
IFA
s.c.
24
Boost #2
IFA
s.c.
35
Boost #3
IFA
i.v.
42
Bleed and test for antibody
45
Boost #4
IFA
i.v.
66+
3-4 week rest followed by i.v. or i.p. boost
Saline/PBS
i.v.
69
fusion 3d after i.v. boost or 4d after i.p. boost
--
i.v.
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