样品处理液配方：
50mM Tris-HCl(pH 6.8)
100mM DTT(or 5% 巯基乙醇)
2% SDS
0.1% 溴酚蓝
10%甘油
⑻ 浓缩胶聚合完全后（30分钟），小心移出梳子。把凝胶固定于电泳装置上，上下槽各加入Tris-甘氨酸电极缓冲液。必须设法排出凝胶底部两玻璃板之间的气泡。
Tris-甘氨酸电极缓冲液：
25mM Tris
250mM 甘氨酸 (pH 8.3)
0.1% SDS
⑼ 按予定顺序加样，加样量通常为上一篇：等电聚焦电泳法测定蛋白质的等电点 下一篇：SDS-PAGE 【UCSF】 共43页: 上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] 39 [40] [41] [42] [43] 下一页
上一篇：等电聚焦电泳法测定蛋白质的等电点 下一篇：SDS-PAGE 【UCSF】
共43页: 上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] 39 [40] [41] [42] [43] 下一页
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