Prowl Lab,Rockefeller University
http://prowl.rockefeller.edu/recipes/sproteas/elastase.htm
SpecificityNot very specific. Cleavage at C-terminal side of G, A, S, V, L and I.
pH optimum8.0 - 8.5
StabilityEnzyme is active in 0.1% SDS. The enzyme rapidly autolyzes. 0.1 mM calcium ions stabilize the enzyme.
RecipeDissolve the substrate at 1-10 g/l in 100 mM ammonium bicarbonate. Add elastase at 1:50 and incubate for 4 hours at 37 C.
Grunnet, I. and Knudsen, J. Medium-chain fatty acid synthesis by goat mamary-gland fatty acid synthetase. The effect of limited proteolysis. Biochem. J. 209(1983)215-222.
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