Prowl Lab,Rockefeller University
http://prowl.rockefeller.edu/recipes/sproteas/endoglu.htm
SpecificityCleaves at E-X and D-X bonds in phosphate buffers and at E-X bonds in ammonium bicarbonate buffers. Cleavage does not occur if X=P.
pH optimum4.0-8.0
StabilityEnzyme is active in 0.2% SDS, 1 M guanidine hydrochloride and 4 M urea. Some activity is retained at up to 100 C. The enzyme is unaffected by the presence or absence of divalent cations and can be used in the presence of EDTA.
RecipeFor cleavage of E-X bonds: Dissolve the substrate in 100 mM ammonium bicarbonate at 10 g/l. Add enzyme 1:30 and incubate for 2-18 hours at 37 C. For cleavage of E-X and D-X bonds: Dissolve the substrate in 100 mM phosphate buffer (pH 7.8) at 10 g/l. Add enzyme 1:30 and incubate for 2-18 hours at 37 C.
Tomasselli, A.G., Frank, R. and Schiltz, E. The complete primary structure of GTP:AMP phosphotransferase from beef heart mitochondria. FEBS Lett. 202(1986)303-307.
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