| Prowl Lab,Rockefeller University
http://prowl.rockefeller.edu/recipes/nproteas/aminom.htm
Specificity
Cleavage does not occur for X-P bonds or at N-blocked amino acids.
pH optimum
7.0 - 9.0
Stability
Enzyme is stabel between pH 3.5 to 11.0.
Recipe
Dissolve substrate at 1-10 g/l in 50 mM ammonium bicarbonate. Add enzyme at 1:50 and remove times aliquots to obtain a sequencing ladder.
Wachsmuth, E.E., Fritze, I., and Pleiderer, G., An amiopeptidase occuring in pig kidney. An improved method of preparation. Physical and enzymic properties. Biochem. 5(1966)169-174.
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