Sefton Lab,Salk Institute
This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviously use fewer or more cells, depending on your needs. Don't skimp too much on the amount of S. aureus bugs that you use for precipitation or you will risk losses.
http://pingu.salk.edu/~sefton/Hyper_protocols/angio.html
