| Decant flow-through.
Spin at high speed to dry column.
Transfer spin unit to fresh eppendorf tube.
Add 30 mL Buffer EB to center of filter and allow to sit 3 minutes at RT.
Spin at 13K rpm for 1 minute.
Repeat elution step again with another 30 mL of Buffer EB.
Pool eluates.
Add 20 mL (20 mg) Cot DNA (Gibco).
Add 420 TE and apply to fresh Microcon-30 filter.
Spin 12,000g to a volume of 29 mL or less.
For 38 mL array hybridization:
| 
