Repeat elution step again.
VI. Hyb. Prep.
Dry down Qia-quick eluate in speed vac.
Bring volume to 18 ul with water and HEPES pH 7.5 so that the final concentration (after SSC and other additions) is 25 mM.
Add: 3.6ul 20X SSC
1.8ul polyA(10mg/ml) and/or other competitor nucleic acid
Optional: filter in Millipore 0.45micron spin column.
Add: 0.54ul 10% SDS.
Incubate reaction at 100° C for 2 min.
Apply to prepared microarray.
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