Heat to 95C for 5min, place on ice for 5min

Labeling

Clean up Labeled Probes

• Prewash Microcon-30 microfilter by adding 450ml miliQ H₂O and spinning for 10 min. @ 12,000 RPM.
• Add 450ml miliQ H₂O to each of the probe samples (or total 500ul). Mix thoroughly by pipetting up and down. Transfer samples to separate Microcon-30 microfilters. (Amicon)
• Spin at 12,000 RPM in microfuge for 10 minutes or until 20-40 ml remains in the filter.
• Add 450 ml miliQ H₂O to the probe and gently mix by pipetting up and down. Be careful not to touch the filter at the bottom of the filtration unit.
• Spin 10 minutes at 12,000 RPM.
• Repeat step 4 , spin 12min to get smaller volume.
• Invert column into a fresh tube and spin 1 minute at maximum speed to recover probe. Carefully measure recovered probe volume if necessary.

Probe can be stored at 4°C or -20°C in dark for further use.

Reagents and Suppliers

Genomic DNA Labeling Protocol

点击：   作者：51protocol收集   来源：  时间： 2007-03-13 　本站论坛

Genomic DNA Labeling Protocol We typically use 0.5ug of E. coli genomic DNA in a labeling reaction for each hybridization. The genomic DNA was fragmented to 500 to 1000bps before labeling. The following protocol should produce enough labeled probe for 8 hybridizations. For labeling 4ug Genomic DNA: DNA Mix