Other Mycoplasma Media
Modified Edward's Media (Kahane)
Base:
| Bacto Heart Infusion Broth |
1.2% |
| Bacto Yeast Extract |
0.5% |
| Bacto Peptone |
0.5% |
| NaCl |
0.025% |
| Na-DNA |
0.002% |
- pH is adjusted to 8 and autoclaved.
- May be stored at room temperature for 4 weeks
ADD (to final conc.)
| K2HPO4 |
0.17% |
| Glucose |
1.0% |
| Thallium Acetate |
0.025% |
| Penicillin |
500u/ml |
| Horse Serum |
10% |
| Na-DNA |
0.0025% |
- pH to 7.8
- 4% culture is added and grown statically at 37°C for 18-20 hours to A260
A7 agar (21)
| Gibco base (A7) |
49.5 g |
| DW |
1237.5 ml |
- pH to 5.5
- Autoclave
| Horse Serum |
300 ml |
| Yeast Extract |
15 ml |
| CVA |
7.5 ml |
| Urea (10%) |
15 ml |
| Cysteine (4%) |
3.75 ml |
| amp., fung., nyst. |
0.5 ml |
| Penicillin G |
1 ml |
10B
- 10B Base
-
| PPLO |
22.1 g |
| DW |
1070 ml |
| Phenol Red |
1.5 ml |
- pH to 5.5
- Dispense into 100 ml bottles with ~ 70ml / bottle and autoclave
- Add/bottle
-
| Horse Serum |
20 ml |
| Yeast Extract |
10 ml |
| CVA |
0.5 ml |
| Cysteine |
0.25 ml |
| Urea |
0.4 ml |
| amp., fung., nyst. |
0.05 ml |
| Penicillin G |
0.1 ml |
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