• Sizing of YAC Clones

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Solutions:

• Yeast Lysis Buffer:
  50 mM EDTA

  1% Sarkosyl

  10 mM Tris pH 8.0

  1 mg/ml Proteinase K

References:

Carle, G.F., and M.V.Olson. (1985). "An electrophoretic karyotype for yeast." PNAS, 82: 3756-3760.

Carle, G.F., and M.V.Olson. (1987). "Orthogonal field alternation gel electrophoresis." In Methods in Enzymology, vol. 155, part F, R. Wu (ed.), Academic Press, San Diego, pp. 468-482.