来自Dr. DE Koshland, Carnegie Institution of Washington
http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/frozen permanents.html
1) grow culture to stationary in YEPD
2) Add 0.8ml of culture to 0.8ml of 30% glycerol in labelled freezer vials
3) vortex and place at -80oC
4) for strains with plasmids, pick a colony from a selective plate, grow to stationary in YEPD and freeze in glycerol. streak out on selective plates
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