27 In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti ..................................... 377 28 Biochemical Assays for the Characterization of DNA Helicases Robert M. Brosh, Jr. and Sudha Sharma ........................................... 397 29 Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA Maureen Biggerstaff and Richard D. Wood ..................................... 417 30 Assaying for the Dual Incisions of Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site Mahmud K. K. Shivji, Jonathan G. Moggs, Isao Kuraoka, and Richard D. Wood ................................................................... 435 31 Analysis of Proliferating Cell Nuclear Antigen (PCNA) Associated with DNA Excision Repair Sites in Mammalian Cells A. Ivana Scovassi and Ennio Prosperi ............................................... 457 32 Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates Jill A. Mello, Jonathan G. Moggs, and Geneviève Almouzni ........... 477
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