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DNA Repair Protocols SECOND EDITION [Humana Press]

点击： 作者：51protocol收集 来源： 时间： 2007-02-21 　本站论坛

METHODS IN MOLECULAR BIOLOGY™ 314 DNA Repair Protocols Mammalian Systems SECOND EDITION
Edited by Daryl S. Henderson
2006 Humana Press Inc. PDF格式，7M，点击此处下载 1 Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines
by Replica Plating
Malgorzata Z. Zdzienicka ..................................................................... 1
2 Complementation Assays Adapted for DNA Repair–Deficient
Keratinocytes
Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente,
Alain Sarasin, and Thierry Magnaldo ............................................... 9
3 Cytogenetic Challenge Assays for Assessment
of DNA Repair Capacities
William W. Au and Salama A. Salama ................................................ 25
4 Evaluating the Delayed Effects of Cellular Exposure
to Ionizing Radiation
Shruti Nagar, James J. Corcoran, and William F. Morgan ................. 43
5 Inhibition of DNA Synthesis by Ionizing Radiation:
A Marker for an S-Phase Checkpoint
Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka ........................ 51
6 Analysis of Inhibition of DNA Replication in Irradiated Cells
Using the SV40-Based In Vitro Assay of DNA Replication
George Iliakis, Ya Wang, and Hong Yan Wang .................................. 61
7 Cytometric Assessment of Histone H2AX Phosphorylation:
A Reporter of DNA Damage
Xuan Huang and Zbigniew Darzynkiewicz ........................................ 73
8 Detection of DNA Strand Breaks by Flow and Laser Scanning
Cytometry in Studies of Apoptosis and Cell Proliferation
(DNA Replication)
Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafuji............. 81
9 In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
George Iliakis and Nge Cheong .......................................................... 95
10 Detection of DNA Double-Strand Breaks and Chromosome
Translocations Using Ligation-Mediated PCR and Inverse PCR
Michael J. Villalobos, Christopher J. Betti,
and Andrew T. M. Vaughan .......................................................... 109 11 Plasmid-Based Assays for DNA End-Joining In Vitro
George Iliakis, Bustanur Rosidi, Minli Wang,
and Huichen Wang ....................................................................... 123
12 Use of Gene Targeting to Study Recombination
in Mammalian Cell DNA Repair Mutants
Rodney S. Nairn and Gerald M. Adair .............................................. 133
13 Gene-Specific and Mitochondrial Repair
of Oxidative DNA Damage
R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr .......... 155
14 Quantitative PCR-Based Measurement of Nuclear
and Mitochondrial DNA Damage
and Repair in Mammalian Cells
Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli,
and Bennett Van Houten .............................................................. 183
15 Measuring the Formation and Repair of DNA Damage
by Ligation-Mediated PCR
Gerd P. Pfeifer .................................................................................. 201
16 Immunochemical Detection of UV-Induced DNA
Damage and Repair
Marcus S. Cooke and Alistair Robson ............................................... 215
17 A Dot-Blot Immunoassay for Measuring Repair
of Ultraviolet Photoproducts
Shirley McCready .............................................................................. 229
18 Quantification of Photoproducts in Mammalian Cell DNA Using
Radioimmunoassay
David L. Mitchell .............................................................................. 239
19 DNA Damage Quantitation by Alkaline Gel Electrophoresis
Betsy M. Sutherland, Paula V. Bennett,
and John C. Sutherland................................................................. 251
20 The Comet Assay: A Sensitive Genotoxicity Test
for the Detection of DNA Damage and Repair
Günter Speit and Andreas Hartmann ............................................... 275
21 Fast Micromethod DNA Single-Strand-Break Assay
Heinz C. Schröder, Renato Batel, Heiko Schwertner,
Oleksandra Boreiko, and Werner E. G. Müller ............................ 287
22 32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC
Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki ..................... 307
23 Electrophoretic Mobility Shift Assays to Study Protein Binding
to Damaged DNA
Vaughn Smider, Byung Joon Hwang, and Gilbert Chu .................... 323 24 Construction of MMR Plasmid Substrates and Analysis
of MMR Error Correction and Excision
Huixian Wang and John B. Hays ...................................................... 345
25 Characterization of Enzymes that Initiate Base Excision Repair
at Abasic Sites
Walter A. Deutsch and Vijay Hegde................................................. 355
26 Base Excision Repair in Mammalian Cells
Yoshihiro Matsumoto ........................................................................ 365

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