Neural Stem Cells Methods and Protocols神经干细胞实验方法

Neural Stem Cells Methods and Protocols

Edited by Tanja Zigova, Paul R. Sanberg, and Juan R. Sanchez-Ramos

全书下载

PART I ISOLATION AND CULTURE OF NSCS
1 Neural Differentiation of Embryonic Stem Cells
K. Sue O’Shea ........................................................................................ 3
2 Production and Analysis of Neurospheres from Acutely
Dissociated and Postmortem CNS Specimens
Eric D. Laywell, Valery G. Kukekov, Oleg Suslov,
Tong Zheng, and Dennis A. Steindler........................................... 15
3 Isolation of Stem and Precursor Cells from Fetal Tissue
Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao ................... 29
4 Olfactory Ensheathing Cells: Isolation and Culture from the Rat
Olfactory Bulb
Susan C. Barnett and A. Jane Roskams ........................................... 41
5 Culturing Olfactory Ensheathing Glia from the Mouse
Olfactory Epithelium
Edmund Au and A. Jane Roskams .................................................... 49
6 Production of Immortalized Human Neural Crest Stem Cells
Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai,
Myung A. Lee, and Jung H. Bang .................................................. 55
7 Adult Rodent Spinal Cord Derived Neural Stem Cells:
Isolation and Characterization
Lamya S. Shihabuddin ........................................................................ 67
8 Preparation of Neural Progenitors from Bone Marrow
and Umbilical Cord Blood
Shijie Song and J. Sanchez-Ramos .................................................. 79
9 Seeding Neural Stem Cells on Scaffolds of PGA, PLA,
and Their Copolymers
Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer ......... 89

PART II CHARACTERIZATION OF NSCS IN VITRO
A. CELLULAR TECHNIQUES
10 Analysis of Cell Generation in the Telencephalic Neuroepithelium
Takao Takahashi, Verne S. Caviness, Jr.,
and Pradeep G. Bhide ................................................................... 101
11 Clonal Analyses and Cryopreservation
of Neural Stem Cell Cultures
Angelo L. Vescovi, Rossella Galli, and Angela Gritti ................... 115
12 Assessing the Involvement of Telomerase in Stem Cell Biology
Mark P. Mattson, Peisu Zhang, and Weiming Fu ........................... 125
13 Detection of Telomerase Activity in Neural Cells
Karen R. Prowse ................................................................................ 137
14 In Vitro Assays for Neural Stem Cell Differentiation
Marcel M. Daadi .................................................................................. 149
15 Electron Microscopy and Lac-Z Labeling
Bela Kosaras and Evan Snyder ....................................................... 157
B. ELECTROPHYSIOLOGICAL TECHNIQUES
16 Techniques for Studying the Electrophysiology of Neurons
Derived from Neural Stem/Progenitor Cells
David S. K. Magnuson and Dante J. Morassutti ............................ 179
C. MOLECULAR TECHNIQUES
17 Fluorescence In Situ Hybridization
Barbara A. Tate and Rachel L. Ostroff ............................................ 189
18 RT-PCR Analyses of Differential Gene Expression
in ES-Derived Neural Stem Cells
Theresa E. Gratsch ............................................................................ 197
19 Differential Display: Isolation of Novel Genes
Theresa E. Gratsch ............................................................................ 213
20 Cell Labeling and Gene Misexpression by Electroporation
Terence J. Van Raay and Michael R. Stark .................................... 223
21 Gene Therapy Using Neural Stem Cells
Luciano Conti and Elena Cattaneo.................................................. 233
22 Modeling Brain Pathologies Using Neural Stem Cells
Simonetta Sipione and Elena Cattaneo .......................................... 245

PART III UTILIZATION/CHARACTERIZATION OF NSCS IN VIVO
A. ENDOGENOUS POOLS OF STEM/PROGENITOR CELLS
23 Activation and Differentiation of Endogenous Neural Stem Cell
Progeny in the Rat Parkinson Animal Model
Marcel M. Daadi .................................................................................. 265
24 Identification of Musashi1-Positive Cells in Human Normal
and Neoplastic Neuroepithelial Tissues by
Immunohistochemical Methods
Yonehiro Kanemura, Shin-ichi Sakakibara,
and Hideyuki Okano ...................................................................... 273
25 Identification of Newborn Cells by BrdU Labeling and
Immunocytochemistry In Vivo
Sanjay S. P. Magavi and Jeffrey D. Macklis ................................... 283
26 Immunocytochemical Analysis of Neuronal Differentiation
Sanjay S. P. Magavi and Jeffrey D. Macklis ................................... 291
27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold