Methods in Molecular Biology VOLUME 185
Embryonic Stem Cells
Edited by Kursad Turksen
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1 Methods for the Isolation and Maintenance of Murine Embryonic
Stem Cells
Marsha L. Roach and John D. McNeish ............................................... 1
2 The Use of Chemically Defined Media for the Analyses of Early
Development in ES Cells and Mouse Embryos
Gabriele Proetzel and Michael V. Wiles .............................................17
3 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
Pierre Savatier, Hélène Lapillonne, Ludmila Jirmanova,
Luigi Vitelli, and Jacques Samarut ................................................. 27
4 Murine Embryonic Stem Cells as a Model for Stress Proteins
and Apoptosis During Differentiation
André-Patrick Arrigo and Patrick Mehlen ..........................................35
5 Effects of Altered Gene Expression on ES Cell Differentiation
Yong Fan and J. Richard Chaillet ....................................................... 45
6 Hypoxic Gene Regulation in Differentiating ES Cells
David M. Adelman and M. Celeste Simon ..........................................55
7 Regulation of Gap Junction Protein (Connexin) Genes and Function
in Differentiating ES Cells
Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko,
and Tetsuro Takamatsu .................................................................... 63
8 Embryonic Stem Cell Differentiation as a Model to Study
Hematopoietic and Endothelial Cell Development
Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa,
and Shin-Ichi Nishikawa ................................................................... 71
9 Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell
Differentiation System
Takumi Era, Stephane Wong, and Owen N. Witte............................. 83
10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage
Development
Toshiyuki Yamane, Takahiro Kunisada, and Shin-Ichi Hayashi ...... 97
11 Differentiation of Embryonic Stem Cells as a Model to Study Gene
Function During the Development of Adipose Cells
Christian Dani ......................................................................................107
12 Embryonic Stem Cell Differentiation and the Vascular Lineage
Victoria L. Bautch ................................................................................117
13 Embryonic Stem Cells as a Model to Study Cardiac,
Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation
Anna M. Wobus, Kaomei Guan, Huang-Tian Yang,
and Kenneth R. Boheler .................................................................127
14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures:
Strategies and Applications
Kishore B. S. Pasumarthi and Loren J. Field ................................. 157
15 Embryonic Stem Cells as a Model for the Physiological Analysis
of the Cardiovascular System
Jürgen Hescheler, Maria Wartenberg, Bernd K. Fleischmann,
Kathrin Banach, Helmut Acker, and Heinrich Sauer ................. 169
16 Isolation of Lineage-Restricted Neural Precursors from Cultured
ES Cells
Tahmina Mujtaba and Mahendra S. Rao ..........................................189
17 Lineage Selection for Generation and Amplification of Neural
Precursor Cells
Meng Li .................................................................................................205
18 Selective Neural Induction from ES Cells by Stromal
Cell-Derived Inducing Activity and Its Potential Therapeutic
Application in Parkinson's Disease
Hiroshi Kawasaki, Kenji Mizuseki, and Yoshiki Sasai ................... 217
19 Epidermal Lineage
Tammy-Claire Troy and Kursad Turksen ......................................... 229
20 ES Cell Differentiation Into the Hair Follicle Lineage In Vitro
Tammy-Claire Troy and Kursad Turksen ......................................... 255
21 Embryonic Stem Cells as a Model for Studying Melanocyte
Development
Toshiyuki Yamane, Shin-Ichi Hayashi, and Takahiro Kunisada... 261
22 Using Progenitor Cells and Gene Chips to Define Genetic Pathways
S. Steven Potter, M. Todd Valerius, and Eric W. Brunskill............269
23 ES Cell-Mediated Conditional Transgenesis
Marina Gertsenstein, Corrinne Lobe and Andras Nagy................. 285
24 Switching on Lineage Tracers Using Site-Specific Recombination
Susan M. Dymecki, Carolyn I. Rodriguez,
and Rajeshwar B. Awatramani ..................................................... 309
25 From ES Cells to Mice: The Gene Trap Approach
Francesco Cecconi and Peter Gruss ................................................335
26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells
Thomas Floss and Wolfgang Wurst .................................................347
上一篇：Fundamental Techniques in Cell Culture 下一篇：Neural Stem Cells Methods and Protocols神经干细胞实验方法
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