Methods in Molecular Biology Single Nucleotide Polymorphisms Methods and Protocols单核苷酸多态性实验方法
Edited by Pui-Yan Kwok, MD, PhD 2003 Humana Press Inc.
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1 SNPs: Why Do We Care? Lisa D. Brooks ............................................................. 1 2 Denaturing High-Performance Liquid Chromatography Andreas Premstaller and Peter J. Oefner .............. 15 3 SNP Detection and Allele Frequency Determination by SSCP Tomoko Tahira, Akari Suzuki, Yoji Kukita, and Kenshi Hayashi .............................................. 37 4 Conformation-Sensitive Gel Electrophoresis Arupa Ganguly........................................................... 47 5 Detection of Mutations in DNA by Solid-Phase Chemical Cleavage Method: A Simplified Assay Chinh T. Bui, Jeffrey J. Babon, Andreana Lambrinakos, and Richard G. H. Cotton ............... 59 6 SNP Discovery by Direct DNA Sequencing Pui-Yan Kwok and Shenghui Duan......................... 71 7 Computational SNP Discovery in DNA Sequence Data Gabor T. Marth........................................................... 85 8 Genotyping SNPs With Molecular Beacons Salvatore A. E. Marras, Fred Russell Kramer, and Sanjay Tyagi ................................................. 111 9 SNP Genotyping by the 5'-Nuclease Reaction Kenneth J. Livak...................................................... 129
10 Genotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide Microarrays Katarina Lindroos, Ulrika Liljedahl, and Ann-Christine Syvänen ........................................ 149 11 Quantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase Minisequencing Charlotta Olsson, Ulrika Liljedahl, and Ann-Christine Syvänen ............................... 167 12 Homogeneous Primer Extension Assay With Fluorescence Polarization Detection Tony M. Hsu and Pui-Yan Kwok ............................ 177 13 Pyrosequencing for SNP Genotyping Mostafa Ronaghi ..................................................... 189 14 Homogeneous Allele-Specific PCR in SNP Genotyping Søren Germer and Russell Higuchi ...................... 197 15 Oligonucleotide Ligation Assay Jonas Jarvius, Mats Nilsson, and Ulf Landegren ............................................... 215 16 Invader Assay for SNP Genotyping Victor Lyamichev and Bruce Neri ......................... 229 17 MALDI-TOF Mass Spectrometry-Based SNP Genotyping Niels Storm, Brigitte Darnhofer-Patel, Dirk van den Boom, and Charles P. Rodi..........241
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