| siLentGene | 点击： 作者：51protocol收集 来源： 时间： 2007-03-19 本站论坛
|
|  | siLentGene™ U6 Cassette RNA Interference System
Promega Corporation
PDF格式文件，点击左侧图标下载查看
I. Description ............................................................................................................1
II. Product Components ...........................................................................................4
III. Primer Design .......................................................................................................5
A. Target Selection...............................................................................................5
B. Downstream Primer Sequence........................................................................5
IV. DNA Amplification Protocol (Part 1) ...................................................................6
A. Amplification Setup..........................................................................................6
B. Amplification Thermal Cycling .........................................................................7
V. DNA Purification Protocol (Part 2) ......................................................................7
VI. Transfection and siRNA Suppression Assay Protocol (Part 3) ........................8
A. General Considerations...................................................................................8
B. Transfection Assay Protocol ............................................................................9
C. Quantitating siRNA Target Gene Suppresion ................................................11
VII. Troubleshooting..................................................................................................12
VIII. Appendix .............................................................................................................14
A. Screening for Optimal Target Sequence........................................................14
B. Determining Optimal Suppression Conditions...............................................15
C. Detection of p53 Suppression by Western Analysis ......................................15
D. References ....................................................................................................17
E. Related Products ...........................................................................................18
上一篇：RNA Interference,Editing, and Modification RNA干扰实验方法的经典著作 下一篇：Current Protocols in Molecular Biology跟分子克隆齐名的实验指导丛书，2004版 | | |
|
|
|
