| E. coli Gene Expression Protocols 大肠杆菌基因表达方法[Humana Press] | | 点击： 作者：51protocol收集 来源： 时间： 2007-03-19 本站论坛 |
|  | Methods in Molecular Biology VOLUME 205
E. coli Gene Expression Protocols大肠杆菌基因表达方法
Edited by Peter E. Vaillancourt
2003 Humana Press Inc.
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内容：
Contributors .................................................................................................... ix
1 Cold-Inducible Promoters for Heterologous Protein Expression
François Baneyx and Mirna Mujacic ................................................... 1
2 Dual-Expression Vectors for Efficient Protein Expression
in Both E. coli and Mammalian Cells
Rebecca L. Mullinax, David T. Wong, Heidi A. Davis,
Kerstein A. Padgett, and Joseph A. Sorge .................................. 19
3 A Dual-Expression Vector Allowing Expression in E. coli
and P. pastoris, Including New Modifications
Angelika Lueking, Sabine Horn, Hans Lehrach,
and Dolores J. Cahill ....................................................................... 31
4 Purification of Recombinant Proteins from E. coli
by Engineered Inteins
Ming-Qun Xu and Thomas C. Evans, Jr. ........................................... 43
5 Calmodulin as an Affinity Purification Tag
Samu Melkko and Dario Neri .............................................................. 69
6 Calmodulin-Binding Peptide as a Removable Affinity Tag
for Protein Purification
Wolfgang Klein ..................................................................................... 79
7 Maltose-Binding Protein as a Solubility Enhancer
Jeffrey D. Fox and David S. Waugh................................................... 99
8 Thioredoxin and Related Proteins as Multifunctional Fusion Tags
for Soluble Expression in E. coli
Edward R. LaVallie, Elizabeth A. DiBlasio-Smith,
Lisa A. Collins-Racie, Zhijian Lu, and John M. McCoy ............ 119
9 Discovery of New Fusion Protein Systems Designed to Enhance
Solubility in E. coli
Gregory D. Davis and Roger G. Harrison ....................................... 141
10 Assessment of Protein Folding/Solubility in Live Cells
Rhesa D. Stidham, W. Christian Wigley, John F. Hunt,
and Philip J. Thomas .................................................................... 155
11 Improving Heterologous Protein Folding
via Molecular Chaperone and Foldase Co-Expression
François Baneyx and Joanne L. Palumbo...................................... 171
12 High-Throughput Purification of PolyHis-Tagged Recombinant
Fusion Proteins
Thomas Lanio, Albert Jeltsch, and Alfred Pingoud ...................... 199
13 Co-Expression of Proteins in E. coli Using Dual Expression Vectors
Karen Johnston and Ronen Marmorstein ...................................... 205
14 Small-Molecule Affinity-Based Matrices
for Rapid Protein Purification
Karin A. Hughes and Jean P. Wiley................................................. 215
15 Use of tRNA-Supplemented Host Strains for Expression
of Heterologous Genes in E. coli
Carsten-Peter Carstens..................................................................... 225
16 Screening Peptide/Protein Libraries Fused to the λ Repressor
DNA-Binding Domain in E. coli Cells
Leonardo Mariño-Ramírez, Lisa Campbell, and James C. Hu ..... 235
17 Studying Protein–Protein Interactions Using a Bacterial
Two-Hybrid System
Simon L. Dove .................................................................................... 251
18 Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli
Cell Surface to Study Protein–Protein Interactions
Zhijian Lu, Edward R. LaVallie, and John M. McCoy .................... 267
19 Use of Inteins for the In Vivo Production of Stable Cyclic Peptide
Libraries in E. coli
Ernesto Abel-Santos, Charles P. Scott,
and Stephen J. Benkovic ............................................................. 281
20 Hyperphage: Improving Antibody Presentation in Phage Display
Olaf Broders, Frank Breitling, and Stefan Dübel ........................... 295
21 Combinatorial Biosynthesis of Novel Carotenoids in E. coli
Gerhard Sandmann ........................................................................... 303
22 Using Transcriptional-Based Systems for In Vivo Enzyme Screening
Steven M. Firestine, Frank Salinas, and Stephen J. Benkovic .... 315
23 Identification of Genes Encoding Secreted Proteins
Using Mini-OphoA Mutagenesis
Mary N. Burtnick, Paul J. Brett, and Donald E. Woods ................ 329
上一篇：Membrane Protein Protocols膜蛋白表达、纯化、鉴定方法[HUMANA PRESS] 下一篇：Protein Purification Protocols Second Edition [HUMANA PRESS]
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