Methods in Molecular Biology VOLUME228
Membrane Protein Protocols Expression, Purification, and Characterization
膜蛋白表达纯化鉴定方法
2003 HUMANA PRESS
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Table of Contents
1. Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli
pp. 3-10
2. Dihydroorotate Dehydrogenase of Escherichia coli
pp. 11-22
3. A General Approach for Heterologous Membrane Protein Expression in Escherichia coli: The Uncoupling Protein, UCP1, as an Example
pp. 23-36
4. Expression of Membrane-Bound Iron-Sulfur Proteins
Schneider, Dirk; Schmidt, Christian L.; Seidler, Andreas
pp. 37-48
5. Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris
Han, Chang-Hoon; Werder, Moritz; von Gustedt, Wilk; Hauser, Helmut
pp. 49-64
6. Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells
Rao, U. Subrahmanyeswara
pp. 65-72
7. Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells
Bosman, Giel J.C.G.M.; van Oostrum, Jenny; Breikers, Githa; Bovee-Geurts, Petra H.M.; Klaassen, Corne H.W.; DeGrip, Willem J.
pp. 73-86
8. Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
Manley, Darren; O'Neil, Joe D.
pp. 89-102
9. Solubilization of Chemokine Receptors from Cell Membranes
Staudinger, Robert; Bandr閟, Juan C.
pp. 103-110
10. A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid: Acyl-Coa Acyltransferase (LAT)
Kerkhoff, Claus; Kaever, Volkhard
pp. 111-128
11. Purification of Omp50, a Minor Porin of Campylobacter jejuni
Bolla, Jean Michel
pp. 131-138
12. Purification of Porins from Mycobacterium smegmatis
Heinz, Christian; Roth, Eva; Niederweis, Michael
pp. 139-150
13. Isolation of the Melanocortin 5 Receptor: From cDNA Sequence to Isolating an Integral Membrane Protein
Clarke, Benjamin L.
pp. 151-162
14. Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography
Hanada, Kentaro; Nishijima, Masahiro
pp. 163-174
15. Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells
Kawasaki, Kiyoshi; Nishijima, Masahiro
pp. 175-186
16. Purification of Pancreastatin Receptor from Rat Liver Membranes
pp. 187-194
17. Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes
Prasad, Rajendra
pp. 195-222
18. Isolation of Lipid Raft-Associated Proteolipids
pp. 223-230
19. Purification of Membrane-Bound Catechol-O-Methyltransferase
pp. 231-238
20. Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane
Wang, Da-Neng; Lemieux, M. Joanne; Boulter, Jonathan M.
pp. 239-256
21. Purification of the Human Erythrocyte PS-Stimulated Mg2 -ATPase: A Putative PS Flippase
Lyles, Jill V.; Cornely-Moss, Kathleen; Smith, Christine M.; Daleke, David L.
pp. 257-270
22. Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes
pp. 271-280
23. Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I
Brown, Nicholas F.
pp. 281-302
24. Crystallization in Lipidic Cubic Phases: A Case Study with Bacteriorhodopsin
Gordeliy, Valentin I.; Schlesinger, Ramona; Efremov, Rouslan; Georg; Heberle, Joachim
pp. 305-316
25. Optical Biosensor Assay Using Retroviral Receptor Pseudotypes
Rucker, Joseph
pp. 317-328
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