Methods in Molecular Biology VOLUME 124
Protein Kinase Protocols 蛋白激酶实验方法
Edited by Alastair D. Reith
HUMANA PRESS
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Table of Contents
1. Protein Kinase-Mediated Signaling Networks: Regulation and Functional Characterization
Reith, Alastair D.
pp. 1-20
2. Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies
Chong, Lisa D.; Daar, Ira O.
pp. 21-37
3. Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins
Ignatoski, Kathleen M. Woods
pp. 39-48
4. Two-Dimensional Phosphoamino Acid Analysis
Blume-Jensen, Peter; Hunter, Tony
pp. 49-65
5. Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases
Tavar? Jeremy M.; Issad, Tarik
pp. 67-85
6. Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry
Craig, A. Grey
pp. 87-105
7. Phosphorylation of Smad Signaling Proteins by Receptor Serine/Threonine Kinases
Souchelnytskyi, Serhiy; R鰊nstrand, Lars; Heldin, Carl-Henrik; Dijke, Peter ten
pp. 107-120
8. Assays for Mitogen-Activated Protein Kinase (MAPK) Subtypes and MAPK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate
LePanse, Rozen; Rampersaud, Navita; Mahadevan, Louis C.
pp. 121-130
9. JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins: Analysis by Phosphotyrosine Blotting and EMSA
Broughton, Nicola; Burfoot, Mark S.
pp. 131-146
10. Assays for Glycogen Synthase Kinase-3 (GSK-3)
Cross, Darren
pp. 147-159
11. Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements
Brooks, Gavin
pp. 161-170
12. Protein Histidine Kinase
Matthews, Harry R.; Chan, Karina
pp. 171-182
13. Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System
Purton, Tracey; Wilkinson, Sandra; Murray, Edward J.
pp. 183-194
14. Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins
Zirngibl, Ralph A.; Greer, Peter
pp. 195-208
15. Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay
Burnham, Mary Rose; DeBerry, Regina; Bouton, Amy H.
pp. 209-220
16. Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries
Di Fiore, Pier Paolo; Fazioli, Francesca
pp. 221-237
17. Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of Receptor Targets (CORT) Strategy
Daly, Roger J.
pp. 239-249
18. Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System
Prigent, Sally A.
pp. 251-270
19. cDNA Expression Cloning and Characterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System
Volpers, Christoph; Lubinus, Manuel; Osborne, Mark A.; Kochan, Jarema P.
pp. 271-293
20. Analysis of SH2 Domain ?Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance
Panayotou, George; Ladbury, John
pp. 295-311
21. Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins
Cheng, Hwai-Jong; Flanagan, John G.
pp. 313-334
22. Isolation and Characterization of "Orphan-RTK" Ligands Using an Integrated Biosensor Approach
Lackmann, Martin
pp. 335-359
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