| Monoclonal Antibody Production | | 点击： 作者：51protocol收集 来源： 时间： 2007-03-19 本站论坛 |
|  | A Report of the
Committee on Methods of Producing Monoclonal Antibodies Institute for Laboratory Animal Research National Research Council
NATIONAL ACADEMY PRESS Washington, DC 1999
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CONTENTS
COMMITTEE ON METHODS OF PRODUCING MONOCLONAL ANTIBODIES................................ iii
PREFACE...............................................................................................................................................VII
CONTENTS.............................................................................................................................................IX
EXECUTIVE SUMMARY........................................................................................................................ 1
INTRODUCTION ..................................................................................................................................... 4
GENERATION OF HYBRIDOMAS: PERMANENT CELL LINES SECRETING MONOCLONAL
ANTIBODIES............................................................................................................................................ 6
IN VITRO PRODUCTION OF MONOCLONAL ANTIBODY .............................................................. 9
BATCH TISSUE-CULTURE METHODS ........................................................................................................... 9
SEMIPERMEABLE-MEMBRANE-BASED SYSTEMS........................................................................................ 10
SCIENTIFIC NEEDS FOR MOUSE ASCITES PRODUCTION OF MAB.......................................... 12
SUMMARY OF ADVANTAGES AND DISADVANTAGES OF IN VITRO AND IN VIVO
METHODS .............................................................................................................................................. 16
ADVANTAGES OF IN VITRO METHODS....................................................................................................... 16
DISADVANTAGES OF IN VITRO METHODS.................................................................................................. 16
ADVANTAGES OF MOUSE ASCITES METHOD.............................................................................................. 17
DISADVANTAGES OF MOUSE ASCITES METHODS....................................................................................... 17
LARGE-SCALE PRODUCTION OF MONOCLONAL ANTIBODIES............................................... 18
MONOCLONAL ANTIBODY PRODUCTION FOR DIAGNOSTIC AND THERAPEUTIC PURPOSES ........................... 18
IN VIVO AND IN VITRO METHODS FOR COMMERCIAL PRODUCTION OF MAB............................................... 19
In Vivo Production ............................................................................................................................... 20
In Vitro Production .............................................................................................................................. 21
REGULATORY REQUIREMENTS.................................................................................................................. 23
ANIMAL-WELFARE ISSUES RELATED TO THE ASCITES METHOD FOR PRODUCING
MONOCLONAL ANTIBODIES ............................................................................................................. 24
AVAILABILITY OF DATA ........................................................................................................................... 24
ANIMAL-WELFARE ISSUES RELATED TO ASCITES METHOD ....................................................................... 24
METHODS FOR MEASURING PAIN OR DISTRESS IN LABORATORY RODENTS ................................................ 25
PRIMING.................................................................................................................................................. 26
ASCITES.................................................................................................................................................. 27
HARVESTING ASCITIC FLUID..................................................................................................................... 29
FEEDER CELL HARVESTING AND SERUM SUPPLEMENTS FOR IN VITRO HYBRIDOMA CULTURE .................... 30
SUMMARY OF ANIMAL WELFARE ISSUES .................................................................................................. 30
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