GTPase Protocols [HUMANA PRESS]

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Table of Contents
1. The GTPase Cycle: How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases
Manser, Edward J.
pp. 003-011
2. Preparation of GTPases for Structural and Biophysical Analysis
Smith, Susan J. M.; Rittinger, Katrin
pp. 013-024
3. Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes
Schmitz, Arndt A. P.; Lucito, Robert; Van Aelst, Linda
pp. 025-043
4. Fluorescence Methods in the Study of Small GTP-Binding Proteins
Ahmadian, Mohammad Reza; Wittinghofer, Alfred; Herrmann, Christian
pp. 045-063
5. Ras and Rac as Activators of Reactive Oxygen Species (ROS)
Archer, Herbert; Bar-Sagi, Dafna
pp. 067-073
6. Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs
Maruta, Hiroshi; He, Hong; Nheu, Thao
pp. 075-085
7. A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays
Zhao, Zhoushen; Lim, Louis
pp. 087-095
8. Determination of the Activity of Rho-Like GTPases in Cells
Malliri, Angeliki; ten Klooster, Jean P.; Olivo, C.; Collard, John G.
pp. 099-109
9. Introduction of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases
Tan, Ivan; Lim, Louis; Leung, Thomas
pp. 111-119
10. Effects of Rho Family GTPases on Cell-Cell Adhesion
Fukata, Masaki; Nakagawa, Masato; Kuroda, Shinya; Kaibuchi, Kozo
pp. 121-128
11. Cell Motility and Invasion Assays
Banyard, Jackie; Symons, Marc
pp. 129-140
12. Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases
Nakanishi, Hiroyuki; Sasaki, Takuya; Takai, Yoshimi
pp. 143-156
13. Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase
Pereira-Leal, Jos?B.; Gomes, Anita Q.; Seabra, Miguel C.
pp. 157-165
14. Preparation of Myristoylated Arf1 and Arf6 Proteins
Randazzo, Paul A.; Fales, Henry M.
pp. 169-179
15. ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins
Pacheco-Rodriguez, Gustavo; Moss, Joel; Vaughan, Martha
pp. 181-189
16. Arf6 and its Role in Cytoskeletal Modulation
Donaldson, Julie G.
pp. 191-198
17. ARF GTPase-Activating Protein 1
Huber, Irit; Cukierman, Edna; Rotman, Miriam; Cassel, Dan
pp. 199-206
18. The Use of Permeabilized Cell Systems to Study Nuclear Transport
Brownawell, Amy M.; Holaska, James M.; Macara, Ian G.; Paschal, Bryce M.
pp. 209-229
19. Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes
Kehlenbach, Ralph H.; Gerace, Larry
pp. 231-245
20. Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton
Lizarraga, Sofia B.; Zheng, Yixian; Wilde, Andrew R.
pp. 247-260