METHODS IN MOLECULAR BIOLOGY Volume 284
Signal Transduction Protocols SECOND EDITION 信号转导实验方法第二版
Edited by Robert C. Dickson ,Michael D. Mendenhall
Humana Press
PDF格式文件，313页，1.6M，点击此处下载
内容：
1. Making Protein Immunoprecipitates
Elaine A. Elion and Yunmei Wang
2. Signal Transduction Inhibitors in Cellular Function
Maofu Fu, Chenguang Wang, Xueping Zhang, and Richard G. Pestell
3. Two-Dimensional Gel Electrophoresis for the Identification of
Signaling Targets
Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing, and Natalie
G. Ahn
4. A High-Throughput Mammalian Cell-Based Transient Transfection
Assay
Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski
5. Determining Protein Half-Lives
Pengbo Zhou
6. Assaying Protein Kinase Activity
Jan Brábek and Steven K. Hanks
7. Comparative Phosphorylation Site Mapping From Gel-Derived
Proteins Using a Multidimensional ES/MS-Based Approach
Francesca Zappacosta, Michael J. Huddleston, and Roland S. Annan
8. Studies of Calmodulin-Dependent Regulation
Paul C. Brandt and Thomas C. Vanaman
9. Measurement of Protein–DNA Interactions In Vivo by Chromatin
Immunoprecipitation
Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing
Wu, and Emery H. Bresnick
10. Characterization of Protein–DNA Association In Vivo by Chromatin
Immunoprecipitation
Laurent Kuras
11. Nonradioactive Methods for Detecting Activation of Ras-Related
Small G Proteins
Douglas A. Andres
12. Nucleocytoplasmic Glycosylation, O-GlcNAc Identification and Site
Mapping
Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart
13. Techniques in Protein Methylation
Jaeho Lee, Donghang Cheng, and Mark T. Bedford
14. Assaying Lipid Phosphate Phosphatase Activities
Gil-Soo Han and George M. Carman
15. Assaying Phosphoinositide Phosphatases
Gregory S. Taylor and Jack E. Dixon
16. Assaying Phospholipase A2 Activity
Christina C. Leslie and Michael H. Gelb
17. Measurement and Immunofluorescence of Cellular Phosphoinositides
Hiroko Hama, Javad Torabinejad, Glenn D. Prestwich, and Daryll B.
DeWald
18. Measuring Dynamic Changes in cAMP Using Fluorescence Resonance
Energy Transfer
Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron,
and Manuela Zaccolo
19. In Vivo Detection of Protein–Protein Interaction in Plant Cells Using
BRET
Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, and Albrecht G.
von Arnim
20. Revealing Protein Dynamics by Photobleaching Techniques
Frank van Drogen and Matthias Peter
21. Assaying Cytochrome c Translocation During Apoptosis
Nigel J. Waterhouse, Rohan Steel, Ruth Kluck, and Joseph A. Trapani
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