| RT-PCR Protocols RT-PCR实验方法 [Humana Press] | 点击： 作者：51protocol收集 来源： 时间： 2007-03-19 本站论坛
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and Fergal O’Gara ........................................................................ 253
PART VIII. RT-PCR IN IMMUNOLOGY
21 Detection of Clonally Expanded T-Cells by RT-PCR-SSCP
and Nucleotide Sequencing of T-Cell Receptor β-CDR3 Regions
Manae Suzuki Kurokawa, Kusuki Nishioka,
and Tomohiro Kato ...................................................................... 267
22 Generation of scFv from a Phage Display Mini-Library Derived
from Tumor-Infiltrating B-Cells
Nadège Gruel, Beatrix Kotlan, Marie Beuzard,
and Jean-Luc Teillaud ................................................................. 281
23 Generation of Murine scFv Intrabodies from B-Cell Hybridomas
Chang Hoon Nam, Sandrine Moutel,
and Jean-Luc Teillaud ................................................................. 301
24 Quantitation of mRNA Levels by RT-PCR in Cells Purified
by FACS: Application to Peripheral Cannabinoid Receptors
in Leukocyte Subsets
Jean Marchand and Pierre Carayon ................................................ 329
PART IX. RT-PCR IN ANTI-SENSE TECHNOLOGY
25 Detection of Anti-Sense RNA Transcripts by Anti-Sense RT-PCR
Michael C. Yeung and Allan S. Lau ................................................. 341
PART X. RT-PCR IN CDNA CLONING
26 RT-PCR in cDNA Library Construction
Vincent Healy ..................................................................................... 349
27 An RT-PCR-Based Protocol for the Rapid Generation of Large,
Representative cDNA Libraries for Expression Screening
Joe O’Connell ..................................................................................... 363
Index ............................................................................................................ 375
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