Gene Knockout Protocols基因敲除实验方法[Humana Press]
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Gene Knockout Protocols基因敲除实验方法[Humana Press]

点击:   作者:51protocol收集   来源:  时间: 2007-03-18  本站论坛
Methods in Molecular Biology VOLUME158 Gene Knockout Protocols 基因敲除实验方法 M. J. Tymms and I. Kola Humana Press Inc.
PDF格式,共437页,4.5M,点击此处下载
内容: 1 Overview Gene Knockouts 2 Isolation and Maintenance of Primate Embryonic Stem Cells 3 Gene Targeting in ES Cells 4 Manipulating Mouse Embryonic Stem Cells 5 Gene Targeting in a Centralized Facility 6 The LoxP/CRE System and Genome Modification 7 Creation and Use of a Cre Recombinase Transgenic Database 8 Choice of Mouse Strains for Gene Targeting 9 Isolation, Microinjection, and Transfer of Mouse Blastocysts 10 Aggregation Chimeras 11 How to Study Pathologic Phenotypes of Knockout Mice 12 Analysis of Hematopoietic Phenotypes in Knockout Mouse Models 13 Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function 14 Influence of Genetic Background on Knockout Mouse Phenotypes 15 Lineage Allocation During Early Embryogenesis 16 Generation of Double-Knockout Embryonic Stem Cells 17 In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes 18 Embryonic Stem Cells in the Study of Hematopoiesis 19 Interferon-Inducible ES Cell Expression Systems …………

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