果蝇细胞系的介绍（Drosophila Cell Lines）-试验方案

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Origin: 3rd instar larvae wing imaginal discs Reference: Peel et al., 1990^{_FAQ_lines.pl#note1">1} Received from: Characteristics: columnar epithelial, adherent, will form multiple layers, conserved signaling pathways Karyotype: Diploid

Drosophila Line 1 (otherwise known as Schneider Line 1) - (DL1)

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Schneider, 1972^{_FAQ_lines.pl#note2">2} Received from: Ranjit Dasgupta-University of Wisconsin-Madison Characteristics: Hemocyte-like gene expression, phagocytic, adherent monolayer of uniformly round, smooth cells Karyotype:

Drosophila Line 2 (otherwise known as Schneider Line 2) - (DL2)

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Schneider, 1972^{_FAQ_lines.pl#note2">2} Received from: Peter Christian Characteristics: Hemocyte-like gene expression, phagocytic, adherent monolayer of uniformly round, smooth cells Karyotype:

Kc (Kc 167)

Origin: Dissociated embryos, 8 - 12 h (F2 ebony x sepia) Reference: Echalier and Ohanessian, 1969³ Received from: Cherbas Characteristics: Hemocyte-like gene expression, phagocytic, uniformly round, clump in sheets, ecdysone responsive into adherent, bipolar spindle-shaped cells Karyotype: up to 90% diploid, XX with one IVth punctiform chromosome

ML-DmBG2

Origin: Dissociated 3rd instar larvae brain and ventral ganglia (y v f mal) Reference: Ui et al, 1994⁴ Received from: Characteristics: acetylcholine, HRP expression, neuronal-like processes Karyotype: Diploid

Schneider's Line S2 - (S2)

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Schneider, 1972² Received from: Invitrogen Characteristics: Hemocyte-like gene expression, phagocytic, semi-adherent in colonies, round, granular cytoplasm Karyotype: female, 60%-80% tetraploid

Schneider's Line S2-(SL2)

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Bruckner et al. 2000⁵ Received from: Steve Cohen Characteristics: Hemocyte-like gene expression, phagocytic, semi-adherent in colonies, round, granular cytoplasm. Karyotype: female, 60%-80% tetraploid

Schneider's Line S3

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Schneider, 1972² Received from: Cherbas Characteristics: adherent, spindle-shaped cells, ecdysone responsive, grow in clumps Karyotype: XX and XY, 90% diploid

Schneider's S2 - (S2*)

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Schneider, 1972² Received from: Maniatis Characteristics: Hemocyte-like gene expression, phagocytic, semi-adherent in colonies, round, granular cytoplasm Karyotype:

Schneider's S2 - (S2-R+)

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Yanagawa, S. et al, 1998⁶ Received from: Yanagawa Characteristics: Hemocyte-like gene expression, phagocytic, adherent, flat cells; Fz+ and Wg-responsive Karyotype:

Schneider's S2 - (S2C)

Origin: Dissociated embryos, near hatching (Oregon R) Reference: Schneider, 1972² Received from: Cherbas Characteristics: Hemocyte-like gene expression, semi-adherent in colonies, round, granular cytoplasm Karyotype:

References:

Peel DJ, Johnson SA, Milner MJ. Tissue Cell. 1990;22(5):749-58.
Schneider I. J Embryol Exp Morphol. 1972 Apr;27(2):353-65.
Echalier, G. and A. Ohanessian. C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 31;268(13):1771-3.
Ui K, Nishihara S, Sakuma M, Togashi S, Ueda R, Miyata Y, Miyake T. In Vitro Cell Dev Biol Anim. 1994 Apr;30A(4):209-16.
Bruckner et al. 2000, Nature 406(6794):411-5
Yanagawa, S., J.S.Lee, A. Ishimoto. J Biol Chem. 1998 Nov 27;273(48)

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果蝇细胞系的介绍（Drosophila Cell Lines）
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注：这里的果蝇细胞系指的来源于国际果蝇基因组资源中心的 Drosophila Cell Lines in the DRSC（Drosophila Genomics Resource Center ）

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