TO PREPARE 1*LIQUID 1. Measrue out 5% less distilled water than desired total volume of medium using a mixing container that is as close to the final volume as possible. 2. Add powdered medium to 15 to 30℃ (room temperature)water with gentle stirring. (Do not heat water) 3. Rinse out inside of package to remove all traces of powder. 4. Add 3.7g of NaHCO3 per liter of medium. 5. Dilute to a desired volume with water. Stir until dissolved. (Do not over-mix) 6. Adjust pH of medium to 0.2-0.3 below desired final working pH* use of IN NaOH or IN HCl is recommended. (Add slowly with stirring) After pH has been adjusted keep container closed until medium is filtered. 7. Sterilize immediately by membrane filtration. (Positive pressure recommended) *pH unite will usually rise 0.1-0.3upon filtration.
