胚胎干细胞培养

• (Bowtell Lab)
     
• (Bowtell Lab)
    
• (PMCI)
     
• (Nagy Lab)
    
• (The Cancer Center at Washington University)
• (PMCI)
  Obtain ES cell from mice embryo for culture
    
• ES Cell Culture and Manipulation (Chisholm Lab)
  Detailed protocol for ES cell manipulation, including Media, plating ES cell, Splitting ES cells, freezing ES cells, transformation of ES cells, expansion of ES cell clones and more...

• (Nagy Lab)
  To produce completely ES cell-derived embryos a clump of ES cells is sandwiched between two tetraploid embryos. For ES cell aggregation chimeras with diploid embryos, one embryo is aggregated with a clump of ES cells. Even in this case, the ES cell internalization is efficient enough to achieve a high level of ES cell contribution.  
     
• (Nagy Lab) 
     
• (Gary Brown)
    
• (Gary Brown)
    
• (PMCI)
      
• (Bowtell Lab)
    
• (Bowtell Lab)
      
• (Nagy Lab)
    
• (Bowtell Lab)
     
• (Bowtell Lab)
    
• (Bowtell Lab)
    
• (Bowtell Lab)
    
• (Bowtell Lab)
    
• (Gary Brown)
    
• In Vitro Differentiation of ES Cells into: (Nagy Lab)
  • Cystic Embryoid Bodies
     
• (PMCI)
    
• (PMCI)
    
• (PMCI)
    
• (LTI)
  very useful information about many aspects of ES cell culture
      
• (Bowtell Lab)