Mitshison Lab, Department of Systems Biology, Harvard Medical School
Segmented and polarity-marked microtubules are very useful for many different types of in vitro assays. Segmented microtubules are microtubules with a bright seed and dim elongated segments on both ends. Polarity marked microtubules are microtubules with a bright seed and a dim elongated segment only on one end -- the plus end. ……
http://mitchison.med.harvard.edu/protocols/polarity.html