Mitshison Lab, Department of Systems Biology, Harvard Medical School
Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live DIC analysis has largely superseded the use of visual fixed time point assays for analyzing microtubule dynamics, spindowns can still be very useful. Procedures for fixing and pelleting microtubules onto coverslips are described here. ……
http://mitchison.med.harvard.edu/protocols/spindown.html
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